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human lbp elisa kit  (Hycult Biotech)


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    Hycult Biotech human lbp elisa kit
    Human Lbp Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 95/100, based on 197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lbp/LBP%2C+Human%2C+ELISA+kit/10__1016_slash_j__isci__2026__117743-164-19-23
    Average 95 stars, based on 197 article reviews
    human lbp elisa kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Clinical Proteomics:

    Article Title: The increase in core body temperature in response to exertional-heat stress can predict exercise-induced gastrointestinal syndrome.
    Article Snippet: Utilizing metadata from existing exertional and exertional-heat stress studies, the study aimed to determine if the exercise-associated increase in core body temperature can predict the change in exercise-induced gastrointestinal syndrome (EIGS) biomarkers and exercise-associated gastrointestinal symptoms (Ex-GIS).. Endurance-trained individuals completed 2 h of running exercise in temperate (21.2-30.0°C) to hot (35.0-37.2°C) ambient conditions (n = 132 trials).. Blood samples were collected preand post-exercise to determine the change in gastrointestinal integrity biomarkers and systemic inflammatory cytokines.

    Article Title: Effects of gut barrier dysfunction during a viral respiratory disease challenge on immune function of feedlot beef calves.
    Article Snippet: .. The Page 14 of 81 ScholarOne, 375 Greenbrier Drive, Charlottesville, VA, 22901 Journal of Animal Science D ow nloaded from https://academ ic.oup.com /jas/advance-article/doi/10.1093/jas/skag117/8651337 by U niversidade Federal de Santa C atarina (U FSC ) user on 23 April 2026 15 272 concentrations of IL-6 (kit #ELB-IL6-1; RayBiotech, Inc., Norcross, GA), TNF-α (kit #ELB273 TNFa-1; RayBiotech, Inc.), IL-10 (kit #ELB-IL10-1; RayBiotech, Inc., Norcross, GA), LBP (kit 274 #HK503; Hycult Biotech, Uden, Netherlands), and SAA (kit #TP-802; Tridelta Development 275 Ltd., Kildare, Ireland) in serum or plasma were determined using bovine-specific commercial 276 sandwich enzyme-linked immunosorbent assay (ELISA) kits. ..

    Biomarker Discovery:

    Article Title: The increase in core body temperature in response to exertional-heat stress can predict exercise-induced gastrointestinal syndrome.
    Article Snippet: Utilizing metadata from existing exertional and exertional-heat stress studies, the study aimed to determine if the exercise-associated increase in core body temperature can predict the change in exercise-induced gastrointestinal syndrome (EIGS) biomarkers and exercise-associated gastrointestinal symptoms (Ex-GIS).. Endurance-trained individuals completed 2 h of running exercise in temperate (21.2-30.0°C) to hot (35.0-37.2°C) ambient conditions (n = 132 trials).. Blood samples were collected preand post-exercise to determine the change in gastrointestinal integrity biomarkers and systemic inflammatory cytokines.

    Translocation Assay:

    Article Title: The increase in core body temperature in response to exertional-heat stress can predict exercise-induced gastrointestinal syndrome.
    Article Snippet: Utilizing metadata from existing exertional and exertional-heat stress studies, the study aimed to determine if the exercise-associated increase in core body temperature can predict the change in exercise-induced gastrointestinal syndrome (EIGS) biomarkers and exercise-associated gastrointestinal symptoms (Ex-GIS).. Endurance-trained individuals completed 2 h of running exercise in temperate (21.2-30.0°C) to hot (35.0-37.2°C) ambient conditions (n = 132 trials).. Blood samples were collected preand post-exercise to determine the change in gastrointestinal integrity biomarkers and systemic inflammatory cytokines.

    Enzyme-linked Immunosorbent Assay:

    Article Title: The increase in core body temperature in response to exertional-heat stress can predict exercise-induced gastrointestinal syndrome.
    Article Snippet: Utilizing metadata from existing exertional and exertional-heat stress studies, the study aimed to determine if the exercise-associated increase in core body temperature can predict the change in exercise-induced gastrointestinal syndrome (EIGS) biomarkers and exercise-associated gastrointestinal symptoms (Ex-GIS).. Endurance-trained individuals completed 2 h of running exercise in temperate (21.2-30.0°C) to hot (35.0-37.2°C) ambient conditions (n = 132 trials).. Blood samples were collected preand post-exercise to determine the change in gastrointestinal integrity biomarkers and systemic inflammatory cytokines.

    Article Title: Immune cell infiltration correlates with intestinal permeability, inflammation, and gastrointestinal symptoms in type 1 diabetes.
    Article Snippet: EndoCAb IgG and IgM were measured by Hycult EndoCAb IgG and IgM Elisa kits (HK504-IGG; HK504-IGM; Hycult Biotech, Uden, The Netherlands). .. LBP was measured using a Hycult LBP Human Elisa kit (HK315-02, Hycult Biotech, Uden, The Netherlands). ..

    Article Title: Dietary fatty acid composition drives neuroinflammation and impaired behavior in obesity.
    Article Snippet: .. Mouse ELISA kits were used for Insulin (ALPCO, USA), Leptin (AssayPro, USA), Adiponectin (AssayPro, USA), LBP (LPS binding protein, HycultBiotech, Netherlands) and Corticosterone (LDN, Germany) according to the manufacturer’s protocols. ..

    Article Title: Effects of gut barrier dysfunction during a viral respiratory disease challenge on immune function of feedlot beef calves.
    Article Snippet: .. The Page 14 of 81 ScholarOne, 375 Greenbrier Drive, Charlottesville, VA, 22901 Journal of Animal Science D ow nloaded from https://academ ic.oup.com /jas/advance-article/doi/10.1093/jas/skag117/8651337 by U niversidade Federal de Santa C atarina (U FSC ) user on 23 April 2026 15 272 concentrations of IL-6 (kit #ELB-IL6-1; RayBiotech, Inc., Norcross, GA), TNF-α (kit #ELB273 TNFa-1; RayBiotech, Inc.), IL-10 (kit #ELB-IL10-1; RayBiotech, Inc., Norcross, GA), LBP (kit 274 #HK503; Hycult Biotech, Uden, Netherlands), and SAA (kit #TP-802; Tridelta Development 275 Ltd., Kildare, Ireland) in serum or plasma were determined using bovine-specific commercial 276 sandwich enzyme-linked immunosorbent assay (ELISA) kits. ..

    Article Title: Lipopolysaccharide-binding protein in follicular fluid is associated with the follicular inflammatory status and granulosa cell steroidogenesis in dairy cows
    Article Snippet: The follicular fluid concentrations of NEFA and BHBA were determined using a clinical automated chemistry analyzer (TBA-120FR; Toshiba Medical Systems Co., Ltd., Tochigi, Japan). .. The follicular fluid concentration of LBP was determined using an ELISA kit (LBP for a wide variety of species; HK503; Hycult Biotech, Wayne, PA, USA), following the manufacturer’s guidelines. ..

    Microbiome:

    Article Title: Clinical and Mechanistic Association Between Intestinal Permeability and the Gut Microbiome in Cirrhosis: Role of Phascolarctobacterium.
    Article Snippet: Zonulin (K5600, Immundiagnostik AG, Bensheim, Germany) and Calprotectin (K6927, Immundiagnostik AG, Bensheim, Germany) were measured in stool. .. LBP (HK315, Hycult Biotechnology, Uden, Netherlands), sCD14 (DC140, R and D Systems, Abingdon, UK), and diamino‐oxidase (DAO) (K8500, Key Summary • Established knowledge on this subject: ◦ In patients with liver cirrhosis, the gut microbiome is altered and contributes to increased intestinal permeability, thereby raising the risk for complications and mortality, while also representing a potential therapeutic target • What are the significant and/or new findings of this study? ..

    Permeability:

    Article Title: Clinical and Mechanistic Association Between Intestinal Permeability and the Gut Microbiome in Cirrhosis: Role of Phascolarctobacterium.
    Article Snippet: Zonulin (K5600, Immundiagnostik AG, Bensheim, Germany) and Calprotectin (K6927, Immundiagnostik AG, Bensheim, Germany) were measured in stool. .. LBP (HK315, Hycult Biotechnology, Uden, Netherlands), sCD14 (DC140, R and D Systems, Abingdon, UK), and diamino‐oxidase (DAO) (K8500, Key Summary • Established knowledge on this subject: ◦ In patients with liver cirrhosis, the gut microbiome is altered and contributes to increased intestinal permeability, thereby raising the risk for complications and mortality, while also representing a potential therapeutic target • What are the significant and/or new findings of this study? ..

    Binding Assay:

    Article Title: Dietary fatty acid composition drives neuroinflammation and impaired behavior in obesity.
    Article Snippet: .. Mouse ELISA kits were used for Insulin (ALPCO, USA), Leptin (AssayPro, USA), Adiponectin (AssayPro, USA), LBP (LPS binding protein, HycultBiotech, Netherlands) and Corticosterone (LDN, Germany) according to the manufacturer’s protocols. ..

    Concentration Assay:

    Article Title: Lipopolysaccharide-binding protein in follicular fluid is associated with the follicular inflammatory status and granulosa cell steroidogenesis in dairy cows
    Article Snippet: The follicular fluid concentrations of NEFA and BHBA were determined using a clinical automated chemistry analyzer (TBA-120FR; Toshiba Medical Systems Co., Ltd., Tochigi, Japan). .. The follicular fluid concentration of LBP was determined using an ELISA kit (LBP for a wide variety of species; HK503; Hycult Biotech, Wayne, PA, USA), following the manufacturer’s guidelines. ..



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    A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors <t>LBP</t> and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - <t>lipopolysaccharide,</t> SD – standard deviation, TER – transendothelial electrical resistance
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    A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors <t>LBP</t> and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - <t>lipopolysaccharide,</t> SD – standard deviation, TER – transendothelial electrical resistance
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    A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors <t>LBP</t> and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - <t>lipopolysaccharide,</t> SD – standard deviation, TER – transendothelial electrical resistance
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    A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors <t>LBP</t> and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - <t>lipopolysaccharide,</t> SD – standard deviation, TER – transendothelial electrical resistance
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    A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors <t>LBP</t> and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - <t>lipopolysaccharide,</t> SD – standard deviation, TER – transendothelial electrical resistance
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    lbps rhodopseudomonas palustris cga009  (ATCC)
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    A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors <t>LBP</t> and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - <t>lipopolysaccharide,</t> SD – standard deviation, TER – transendothelial electrical resistance
    Lbps Rhodopseudomonas Palustris Cga009, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors LBP and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance

    Journal: Respiratory Research

    Article Title: The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS

    doi: 10.1186/s12931-026-03928-4

    Figure Lengend Snippet: A HUVECs were stimulated with ECVE nic or control (EBM-2) for 12 hours (incubation). Then, 100 ng/ml LPS with co-factors LBP and CD14 or co-factors alone (sham) were added and incubated for another 18 hours. Created in BioRender. Stahl, K. (2026) https://BioRender.com/nu4iwxz . B Angpt-2 concentrations in the supernatant were measured by ELISA when LPS was added as well as 12 and 18 hours after LPS or sham stimulation and are shown over time. Each group consisted of n =6. Mixed ANOVA with according post-tests were performed. C 18 hours after stimulation with LPS or sham Angpt-2 mRNA transcription was measured by qPCR. Each group consisted of n =9. D Additionally, the Angpt-2/Tie2 mRNA ratio is shown after initial ECVE nic incubation and 18 hours of LPS or sham stimulation. ECVE nic / sham consisted of n=8, all other groups consisted of n =9. Groups were analyzed by Kruskal-Wallis test with post-hoc Dunn test. E TER was measured at different time points by an ECIS system. HUVECs were pre-incubated with ECVE nic ( n =7) or control ( n =8) for 12 hours. Then half of the medium was changed and cells were stimulated with LPS. Results are shown as dot plots with mean ± SD. A linear mixed-effects model was used for analysis. P -values < 0.05 are considered significant. Abbreviations: Angpt-2 - Angiopoietin-2, ECIS - Electric cell-substrate impedance sensing, ECVE – e-cigarette vapor extract, HUVECs – human umbilical vein endothelial cells, LPS - lipopolysaccharide, SD – standard deviation, TER – transendothelial electrical resistance

    Article Snippet: Unless specified otherwise, cells were stimulated with 100 ng/ml lipopolysaccharide (LPS) from E. coli serotype O111:B4 (L4130, Sigma-Aldrich, St. Louis, MO) or vehicle (EBM-2), 100 ng/ml lipopolysaccharide binding protein (LBP) (870-LP-025, R&D Systems, Minneapolis, MN), 100 ng/ml CD14 (SRP3149, Sigma–Aldrich, St. Louis, MO), 500 ng/ml Angpt-1 (923-AN/CF, R&D Systems, Minneapolis, MN), electronic cigarette vapor extract (ECVE, see below) and/or different concentrations (e.g. 0.3, 1, 3–10 μg/ml) of the Tie2 agonist PMC-403 (PharmAbcine, Daejeon, Republic of Korea).

    Techniques: Control, Incubation, Enzyme-linked Immunosorbent Assay, Analysis, Electric Cell-substrate Impedance Sensing, Standard Deviation